The role of vermicompost on yield and alkaloid concentration of fenugreek

Abstract

The current study was conducted to investigate the effect of different levels of vermicompost (0, 6, 12 and 18 g.pot-1 ), peat-moss (12mg.pot-1 ) and DiAmmonium Phosphate (DAP) (1g.pot-1 ) on some yield components (length, No. and weight of pods and No. and weight of seeds) and alkaloids concentration in seed of fenugreek according to the complete randomized design with six replicates. The study was achieved by using high-performance liquated chromatography (HPLC) technology. The analysis of HPLC data shows that the four alkaloids (choline, trigonelline, carpaine and gentianine) have been separated and purified. The result showed a significant difference between different fertilizer levels on yield components and alkaloid concentration, and treatment 12g.pot-1 gives a high value of the studied characteristics mentioned above. Also, results showed that seeds are rich in trigonelline. Keywords: alkaloids, vermicompost, trigonelline and fenugreek