Abstract
ObjectiveTo examine the biochemical and histopathological renal effects of ischemia/reperfusion (I/R) injury using a ruptured abdominal aortic aneurysm (RAAA) model in rats and to investigate the potential protective effects of whortleberry (Vaccinium myrtillus).MethodsThirty-two male Sprague-Dawley rats were randomly assigned into four groups - control, sham (I/R+glycerol), I/R, and I/R+whortleberry. Midline laparotomy alone was performed in the control group. Atraumatic abdominal clamps were attached under anesthesia to the abdominal aorta beneath the level of the renal artery in the groups subjected to I/R. Sixty-minute reperfusion was established one hour after ischemia. The sham group received five intraperitoneal doses of glycerol five days before I/R. The I/R+whortleberry group received a single intraperitoneal 50 mg/kg dose diluted with saline solution five days before I/R. All animals were finally euthanized by cervical dislocation following 60-min reperfusion.ResultsIncreases were observed in malondialdehyde (MDA) levels and tubular necrosis scores (TNS) in thin kidney tissues and in numbers of apoptotic renal tubule cells, together with a decrease in glutathione (GSH) levels, in sham and I/R groups. In contrast, we observed a decrease in MDA levels, TNS, and numbers of apoptotic renal tubule cells, and an increase in GSH levels with whortleberry treatment compared to the I/R group.ConclusionOur findings suggest that whortleberry may be effective against acute kidney injury by reducing oxidative stress and apoptosis.
Highlights
Ruptured abdominal aortic aneurysm (RAAA) is one of the most important emergencies in cardiovascular surgery, with mortality rates of 40-75%[1]
Increases were observed in malondialdehyde (MDA) levels and tubular necrosis scores (TNS) in thin kidney tissues and in numbers of apoptotic renal tubule cells, together with a decrease in glutathione (GSH) levels, in sham and I/R groups
We observed a decrease in MDA levels, TNS, and numbers of apoptotic renal tubule cells, and an increase in GSH levels with whortleberry treatment compared to the I/R group
Summary
Ruptured abdominal aortic aneurysm (RAAA) is one of the most important emergencies in cardiovascular surgery, with mortality rates of 40-75%[1]. Hemorrhagic shock arises from lower body ischemia and reperfusion develops in association with aortic clamping during surgical treatment of RAAA[2]. Bleeding during surgical treatment must be stopped immediately and hemodynamics rapidly restored by applying cross-clamps to the aorta[3]. Declamping shock may occur in association with reperfusion of the lower body when the repair is completed and the aortic clamps are removed. Reperfusion injury develops, and the resulting reactive oxygen radicals (ROS) further exacerbate systemic inflammatory response syndrome. Distant organ damage occurring when hemodynamics cannot be rapidly adjusted and in the presence of severe reperfusion injury increases perioperative mortality and morbidity[1-3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
