The role of \u03b22 integrin in dendritic cell migration during infection

Tarfa Altorki,Andrew Brass,Sheena Cruickshank,Werner Muller

doi:10.1186/s12865-020-00394-5

Tarfa Altorki, Andrew Brass + Show 2 more

Open Access

https://doi.org/10.1186/s12865-020-00394-5

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Abstract

BackgroundDendritic cells (DCs) play a key role in shaping T cell responses. To do this, DCs must be able to migrate to the site of the infection and the lymph nodes to prime T cells and initiate the appropriate immune response. Integrins such as β2 integrin play a key role in leukocyte adhesion, migration, and cell activation. However, the role of β2 integrin in DC migration and function in the context of infection-induced inflammation in the gut is not well understood. This study looked at the role of β2 integrin in DC migration and function during infection with the nematode worm Trichuris muris. Itgb2tm1Bay mice lacking functional β2 integrin and WT littermate controls were infected with T. muris and the response to infection and kinetics of the DC response was assessed.ResultsIn infection, the lack of functional β2 integrin significantly reduced DC migration to the site of infection but not the lymph nodes. The lack of functional β2 integrin did not negatively impact T cell activation in response to T. muris infection.ConclusionsThis data suggests that β2 integrins are important in DC recruitment to the infection site potentially impacting the initiation of innate immunity but is dispensible for DC migration to lymph nodes and T cell priming in the context of T. muris infection.

Highlights

Dendritic cells (DC) play a crucial role in orchestrating T cell responses via their ability to prime and activate naïve T cells
Itgb2mut mice have a reduction in DC and macrophage recruitment to the infection site in response to T. muris infection To determine whether the lack of functional β2 integrin affected the migration of DCs and macrophages to the colon in response to T. muris infection, immunohistochemistry sections from littermate WT controls and Itgb2mut colons were examined in before infection (Day 0) tissue and at Day 19-post infection (p.i) with T. muris
This data suggests that a lack of functional β2 integrin affects the recruitment of macrophages to the site of infection

Summary

Introduction

Dendritic cells (DC) play a crucial role in orchestrating T cell responses via their ability to prime and activate naïve T cells. Β2 integrin plays a crucial role in several immune cell functions such as facilitating intracellular signaling cascades, mediating cytoskeletal rearrangement, recruitment to lymphoid organs and inflamed tissues [8, 9] and adhesion, migration and activation of T cells [7, 10,11,12,13]. DCs must be able to migrate to the site of the infection and the lymph nodes to prime T cells and initiate the appropriate immune response. Integrins such as β2 integrin play a key role in leukocyte adhesion, migration, and cell activation. Itgb2tm1Bay mice lacking functional β2 integrin and WT littermate controls were infected with T. muris and the response to infection and kinetics of the DC response was assessed

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Results

Conclusion