The role of tumor necrosis factor-related apoptosis-inducing ligand, death receptor 4 and decoy receptor 1 during follicle development in polycystic ovary syndrome rats

Abstract

To investigate the role of necosis factor-related apoptosis-inducing ligand (TRAIL), death receptor 4 (DR4) and decoy receptor 1(DcR1) during follicle development in polycystic ovary syndrome rats. Experiment research. Using Sodium Prasterone Sulfate (the sodium sulfate sal of dehydroepiandrosterone) induced PCOS rat model, detected the expression of TRAIL, DR4 and DcR1 in granulosa cells within polycystic ovary syndrome rats through the immunhistochemical staining, reverse transcription polymerase chain reaction (RT-PCR) analysis and western blot analysis. 1. Immunohistochemical analyses showed, the expression of TRAIL in granulosa cells were significantly stronger in antral follicles from the PCOS rats than from the control rats (P<0.05), the expression of TRAIL in granulosa cells were not significantly difference in preantral follicles in two groups (P>0.05), there were no positive staining of TRAIL in granulosa cells of primordial follicles in either group.RT-PCR and western blot analysis showed, the expression of TRAIL mRNA and TRAIL protein were significantly increased in granulosa cells from the PCOS rats than from the control rats (P<0.01). 2. Immunohistochemical analyses showed, the expression of DR4 in granulosa cells were not significantly difference in preantral and antral follicles in two groups (P>0.05), there were no positive staining of DR4 in granulosa cells of primordial follicles in either group.RT-PCR and western blot analysis showed, the expression of DR4 mRNA and DR4 protein were not significantly different in granulosa cells in two groups (P>0.05). 3. Immunohistochemical analyses showed, the expression of DcR1 in granulosa cells were significantly weaker in antral follicles from the PCOS rats than from the control rats (P<0.01), the expression of DcR1 in granulosa cells were not significantly difference in preantral follicles in two groups (P>0.05), there were no positive staining of DcR1 in granulosa cells of primordial follicles in either group. RT-PCR and western blot analysis showed, the expression of DcR1 mRNA and DcR1 protein were significantly decreased in granulosa cells from the PCOS rats than from the control rats (P<0.01). TRAIL, DR4 and DcR1 played a role in regulation the apoptosis of granulose cells during follicle development in PCOS rats. The abnormal expression of TRAIL, DR4 and DcR1 in granulosa cells may be one of the modulation mechanism that induced the failure during follicle development in PCOS.