The role of transient receptor potential ankyrin 1 (TRPA1) receptors in the H2S-evoked calcitonin gene-related peptide (CGRP) release from isolated rat trachea

Abstract

Event Abstract Back to Event The role of transient receptor potential ankyrin 1 (TRPA1) receptors in the H2S-evoked calcitonin gene-related peptide (CGRP) release from isolated rat trachea G. Pozsgai1, T. Bagoly1, Z. Hajna1, M. Boros1, Z. Helyes1, J. Szolcsányi1 and E. Pintér1* 1 University of Pécs, Department of Pharmacology, Hungary TRPA1 ion channels are expressed by capsaicin-sensitive peptidergic primary sensory neurons. TRPA1 activators, such as allylisothiocyanate (mustard oil), airway irritants, cold temperature stimulate these receptors, thus causing inflammatory neuropeptide release (SP, CGRP) and subsequent airway neurogenic inflammation. It has been established that hydrogen sulfide (H2S) like NO exerts neuromediator function and stimulates the capsaicin-sensitive sensory neurons. The aim of the present study was to investigate the effect of H2S on CGRP release from isolated tracheae comparing the action of the TRPA1 agonist mustard oil. Isolated rat tracheae were perfused in organ bath (37 oC, pH 7.2) containing oxygenized Krebs solution. H2S donor NaHS and mustard oil were applied in different doses to elicit neurotransmitter release. CGRP concentrations of the perfusion fluid were determined by radioimmunoassay. Selective TRPA1 antagonist HC030031 was added into the incubation medium to test the involvement of TRPA1 receptors in the CGRP release. Both NaHS and mustard oil induced dose dependent peptide release from the tracheal tissue which was inhibited by the TRPA1 antagonist. On the basis of these data we conclude that H2S may induce neurogenic airway inflammation by activation of TRPA1 ion channels. Acknowledgements OTKA CNK77793, NNF78917 Keywords: Neurophysiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neurophysiology Citation: Pozsgai G, Bagoly T, Hajna Z, Boros M, Helyes Z, Szolcsányi J and Pintér E (2011). The role of transient receptor potential ankyrin 1 (TRPA1) receptors in the H2S-evoked calcitonin gene-related peptide (CGRP) release from isolated rat trachea. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00022 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. E. Pintér, University of Pécs, Department of Pharmacology, Pécs, Hungary, erika.pinter@aok.pte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers G. Pozsgai T. Bagoly Z. Hajna M. Boros Z. Helyes J. Szolcsányi E. Pintér Google G. Pozsgai T. Bagoly Z. Hajna M. Boros Z. Helyes J. Szolcsányi E. Pintér Google Scholar G. Pozsgai T. Bagoly Z. Hajna M. Boros Z. Helyes J. Szolcsányi E. Pintér PubMed G. Pozsgai T. Bagoly Z. Hajna M. Boros Z. Helyes J. Szolcsányi E. Pintér Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.