The role of tip-localized mitochondria in hyphal growth

Abstract

Hyphal tip-growing organisms have a high density of tip-localized mitochondria which maintain a membrane potential based on Rhodamine 123 fluorescence, but do not produce ATP based on the absence of significant oxygen consumption. Two possible roles of these mitochondria in tip growth were examined: Calcium sequestration and biogenesis, because tip-high cytoplasmic calcium gradients are a common feature of tip-growing organisms, and the volume expansion as the tip extends would require a continuous supply of additional mitochondria. Co-localization of calcium-sensitive fluorescent dye and mitochondria-specific fluorescent dyes showed that the tip-localized mitochondria do contain calcium, and therefore, may function in calcium clearance from the cytoplasm. Short-term inhibition of DNA synthesis or mitochondrial protein synthesis did not affect either tip growth, or mitochondrial shape or distribution. Therefore, mitochondrial biogenesis may not occur from the tip-localized mitochondria in hyphal organisms.