Abstract

The functional folding of membrane proteins is critical to cellular homeostasis and increasingly has been shown to depend on physical properties of membranes present. Cell-free translation systems allow for the co-translational folding of membrane proteins into controlled membrane compositions in vitro and enables assessment of how membrane properties influence protein folding. Model membranes that incorporate non-natural amphiphiles present a platform to investigate this relationship by introducing controlled physical features that can be later validated with biological components.

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