The role of the HLA-complex genes in genesis of human idiopathic recurrent pregnancy loss

Abstract

Aim.To study the immunogenetic prerequisites of idiopathic recurrent spontaneous pregnancy losses in humans of allo- and autoimmune genesis. Methods. PCR-SSP (polymerase chain reaction with sequence-specific primers). Results. A comprehensive analysis of the distribution and frequency of allelic variants of the HLA-DRB1, HLA-DQA1, HLA-DQB1 genes testifies to an increased risk of recurrent pregnancy losses with a total homology of 50 % or more in couples and presence of the DQ2.5 genotype in a woman. The OR indicates that the risk of idiopathic recurrent spontaneous pregnancy loss increases 2.68 times when the homology of the couples is 50 % or more for two loci (HLA-DRB1, HLA-DQA1) and 12.8 times when the homology is 50 % or more for by three loci (HLA-DRB1, HLA-DQA1, HLA-DQB1), and if the DQ2.5 genotype is present in women, this risk increases 4 times. Conclusions. HLA genotyping of married couples with a history of spontaneous pregnancy loss is important for preconceptional prevention of recurrent pregnancy losses.