Abstract
Purified yeast phenylalanyl tRNA synthetase (PRS) can aminoacylate highly purified E. , coli tRNA M Met and E. , coli tRNA IIu, but cannot aminoacylate highly purified E. , coli tRNA 3 Gly, E. , coli tRNA His and yeast tRNA Lys. It was previously proposed that a group of nine nucleotides comprising the region adjacent to the dihydrouridine loop were directly involved in the PRS recognition site. In addition to confirming this hypothesis, we now show that a second region of the tRNA molecule, consisting of the fourth nucleotide from the 3'end, is also directly involved in the PRS recognition site. This synthetase has an absolute requirement for adenosine at this location.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
