Abstract
ObjectiveSurvivin is a member of the inhibitor of apoptosis protein family, which uniquely promotes mitosis and regulates apoptosis in cancer cells. Recent studies have demonstrated that survivin also expresses in several normal adult cells. In the present study, we aimed to investigate the function of survivin in the terminally differentiated epithelial cells, podocytes.MethodsSurvivin expression and location were detected by Quantitative Real-Time PCR, western blot and fluorescence confocal microscopy methods in normal and injured mouse podocytes. Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), transfected with survivin siRNA to down-regulate survivin expression, or with survivin plasmid to transiently over-express survivin.ResultsIn podocytes, PAN stimulated expressions of survivin and the apoptosis related molecule caspase 3. Knockdown of survivin expression by siRNA increased the activation of caspase 3, induced podocyte apoptosis and remarkable rearrangement of actin cytoskeleton. Moreover, over-expression of survivin inhibited PAN-induced podocyte apoptosis and cytoskeleton rearrangement.ConclusionOur data provides the evidence that survivin plays an important role in protecting podocytes from apoptosis induced by PAN. The mechanism of survivin related anti-apoptosis may, at least partially, be through the activation of caspase 3.
Highlights
Survivin, is a member of inhibitor of apoptosis family, and is the strongest inhibitor of apoptosis factor identified so far [1,2]
Survivin Expression Was Increased in Podocytes after puromycin aminonucleoside (PAN) Treatment
We investigated survivin expression in cultured podocytes treated with puromycin aminonucleoside (PAN)
Summary
Survivin (gene name Birc5), is a member of inhibitor of apoptosis family, and is the strongest inhibitor of apoptosis factor identified so far [1,2]. Various studies have demonstrated that survivin is over-expressed in cancer tissues/cells [4,5,6,7,8,9], and the higher expression of survivin plays a significant role in the inhibition of apoptosis [10,11,12,13]. Survivin over-expression inhibited ethanol-induced gastric epithelial cells apoptosis [28]. In 2013, Terasaki et al reported that survivin inhibited apoptosis of human lung epithelial cells in acute lung injury, partly by interfering with effector caspases [29]. In our previous studies [36], survivin was found to be significantly up-regulated in podocytes of rats with experimental puromycin aminonucleoside (PAN) nephropathy. We investigated the role of survivin in the podocytes injured by PAN
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