The role of suppressor of cytokine signaling-1 in the differentiation of T cells induced by dendritic cells

Abstract

Objective To investigate the role of suppressor of cytokine signaling-1 ( SOCS1 ) on differentiation of T cells induced by dendritic cells (DCs) in vitro.Methods The human monocyte-derived DCs were induced in the presence of cytokine recombined human granulocyte macrophage-colony stimULating factor (GM-CSF) (1000 U/ml) and interleukin (IL)-4 (500 U/ml).The CDs treated with butyrate (1 mmol/L),lipopolysaccharide (LPS) (1 mg/L),and the cocktail of cytokines [ tumor necrosis factor (TNF)-α,IL-6,IL-1β,PGE2] were detected for surface markers,IL-10,IL-12p40 production and T cells stimulatory proliferation capacity.SOCS1 expression was detected by using Western blotting and real-time polymerase chain reaction (PCR).Results The expression of SOCS1 protein in DCs induced with butyrate was (1.61 ± 0.22),which was increased significantly as compared with cocktail group,LPS group,and control group (P＜0.05).The expression of SOCS1 mRNA in butyrate group was (1.58 ±0.11 ),which was increased significantly as compared with LPS group and cocktail group (P ＜ 0.05 ).High expression of SOCS1 in DCs in butyrate group down-regulated the ability of T-cell stimulation ( 1.53± 1.04) and the production of IL-12 ( 142.79 ± 15.61 ),and promoted IL-10 production (3.29 ±0.21 ).Conclusion The expression of SOCS1 on DCs is the key factor to affect T cell differentiation and induce T cell anergy. Key words: Dendritic cell; Suppressor of cytokine signaling-1 ; T lymphocyte