Abstract
The development of oral biofilm consisting of early settlers and late settlers in the presence and absence of sucrose using a simulated mouth model was investigated. The experiments were carried out by growing the early settlers' biofilm consisti ng of Strep. mitis and Strep. sanguinis and late settlers' biofilm of Strep. mutans in the simulated mouth system. Experiments using t hree different nutrient conditions (A, B and C) were carried out. In A, sterile saliva was used as the nutrient sourc e in which the early settlers were suspended and pumped into the simulated mouth system and allowed to grow for 24 hrs. In B, 1% of BHI broth was added to the sterile saliva three times a day at 6 hrs interval to demonstrate the effect of glucose on th e development of the biofilms. In C, the 1% BHI was supplemented with 1% sucrose in order to show the e ffect of glucose in the presence of sucrose on the development of the biofilm. The experiments were subsequently repeated with the late settlers. In all the experiments, A serves as the control where the nutrient source is minimal. The data collected were analyzed using Student t-test. From the results obt ained, it was observed that both glucose and sucros e enhanced the development of early and late settlers ' biofilms. The growth of late settlers' biofilm however, was found to be significantly higher in th e presence of sucrose (93x10 6 CFU mL ¯ 1 ) compared to glucose (66x10 6 CFU mL ¯ 1 ) with a p value <0.05.
Highlights
Sucrose yields one equivalent of glucose and one equivalent of fructose upon hydrolysis
Sucrose has been considered to be associated with dental caries
Sucrose can be utilized by extracellular bacterial enzymes for the formation of extracellular polysaccharides in dental plaque[1,2,3,4]
Summary
Sucrose yields one equivalent of glucose and one equivalent of fructose upon hydrolysis. Sucrose has been considered to be associated with dental caries. Sucrose can be utilized by extracellular bacterial enzymes for the formation of extracellular polysaccharides (glucans and fructans) in dental plaque[1,2,3,4]. The formation of glucan is catalyzed by glucosyltransferase (GTF) and fructan by fructosyltransferase (FTF)[5,6,7]. Glucan plays a role in plaque formation as well as facilitates the bacterial attachment to the tooth surface[8,9,10]. Fructan contributes to the virulence of the biofilm by acting as binding sites for the adhesion of Strep. Sucrose consumption can result in a decrease in pH in both nonstarved and starved biofilms of Strep. Sucrose consumption can result in a decrease in pH in both nonstarved and starved biofilms of Strep. mutans[11]
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