Abstract
The effect of the l(1)su(f) ts67g mutation and ecdysterone on the expression of glue polypeptide genes in Drosophila melanogaster has been studied. Using cloned DNA harboring glue protein genes Sgs3, Sgs4, Sgs7, and Sgs8, and Northern blot hybridization we show that no or extremely reduced accumulation of RNAs from these genes occurs in mutant larvae shifted to 30°C before and through 70 h after oviposition. On the other hand, a shift-up at 75 h or later results in the production of nearly normal amounts of these RNAs 48 h after shift-up. The results indicate functions of the su(f) gene other than suppression of forked bristles, namely that it is essential early in the third instar for the later transcription of the glue polypeptide genes. We also show that administration of exogenous ecdysterone to hormone deficient larvae induces transcription of Sgs3, Sgs4, Sgs7, and Sgs8 RNAs.
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