The role of Src family kinases in starfish egg fertilisation

Abstract

A common early feature in the activation of all eggs during fertilisation is an increase in the level of intra-cellular free calcium (Ca2+) that, in most species, propagates as a wave across the egg (reviewed in Strieker, 1999). In echinoderms, this Ca2+ release is the result of a signal transduction cascade that requires phospholipase Cγ (PLCγ)-mediated production of inositol trisphosphate (IP3) (Carroll et al., 1997, 1999). PLCγ is most commonly regulated by tyrosine phosphorylation (Rhee & Bae, 1997), indicating that a tyrosine kinase is a likely upstream regulator of PLCγ enzymatic activity at fertilisation. In support of this hypothesis, an increase in tyrosine kinase activity and an increase in tyrosine-phosphorylated proteins at fertilisation has been observed in echinoderm eggs (Satoh & Garbers, 1985; Ciapa & Epel, 1991; Kinsey, 1997). Moreover, the tyrosine kinase inhibitors genistein (Shen et al., 1999) and PP1 (Abassi et al., 2000) have been used to show that in sea urchin eggs a tyrosine kinase activity is required for normal Ca2+ release in response to fertilisation.In eggs of the starfish Asterina miniata, a Src-type tyrosine kinase has been identified as a potential regulator of PLCγ activity at fertilisation (Giusti et al., 1999a). This kinase exhibits a rapid fertilisation-dependent association specifically with the Src Homology 2 (SH2) domains of PLCγ. Moreover, the timing of this association correlates with an increase in the tyrosine kinase activity bound to the PLCγ SH2 domains, and neither the Src kinase nor the associated kinase activity was observed to associate with the PLCγ SH2 domains after treating eggs with the calcium ionophore A23187 (Giusti et al., 1999a). These data identify an egg Src family kinase as a potential upstream regulator of PLCγ during starfish egg fertilisation.