The Role of Silver (Ag) Nanoparticles synthesis by Penicillium spp against the Toxicity of Echinococcus Granulosus in Adult Albino Male Rats

Abstract

The aim of the study was to synthesize Ag nanoparticles by using filamentous fungusPenicillium sp. The fungal culture was isolated from the soil samples collected from agriculture fields in Kirkuk city. The synthesis of silver nanoparticles was investigated by X-ray difraction peaks were measured at (101), (200) and (202) respectively, and scanning electron microscopy .The present study was designed to indicated the role of Ag nanoparticles synthesis by Penicillium spp against toxicity of Echinococcus granulosus. The present study used twenty adult albino male rats that distributed at random to following teams (each group consist five rats); management group received ad libidium, second group injected with 2,5 X 103 of Echinococcus granulosus protoscolices third group injected with protoscolices and treatd with 50 mg/kg AgNanoPs, fourth group injected with protoscolices and treatd with 100mg/kg AgNanoPs. The results show high important exaggerated (P < 0.05) in levels of MDA (malonedialdehyied) and important decrease (P < 0.05) in levels of glutathione (GSH) and catalasce compared with management group. While, after used AgNPs with Echinococcus granulosus, the results indicated non-significant changes (P < 0.05) in MDA, GSH and catalse also showed non-significant changes (P < 0.05) compared with control group. histological study show decrease in numbers of spermatogonia and spermatocytes with absent of spermatid. While, after using AgNPs the testis in third and fourth groups appear semi-normal. It had been ended that AgNanoPs has been potential role against tocxicity of Echinococcus granulosus in rats male.