The Role of Rotavirus in Exacerbated Ulcerative Colitis

Abstract

The Ulcerative colitis disease course is characterized by exacerbations and remissions. Studies of the roleof viral composition within patients with changing disease activity are currently deficient. The rotavirusgastroenteritis is an important cause of morbidity and mortality worldwide. This study aimed to identifythe associated role of Rotavirus during exacerbation of ulcerative colitis. A Fifteen (50) stool and biopsysamples from patients with exacerbated colitis were collected at different age groups and gender. Thesesamples were taken from Morjan Hospital Consultant Gastroenterology and Liver Center, and the lmamSadiq Hospital during the period from October to April 2019. The stool samples were screened for Rotavirusby using Rapid CERtest Rotavirus Kit,PCR The occurrences of UC and age revealed that the age group <25year show ten with UC 83% followed by the age group (26-35) which give seven with UC 70%. The relationbetween existence of UC and gender shown that the positive among male were (20) of patients UC 71%while negative (8) among female the positive were 17 (77%)the results statistically non- significant. Thefrequency of rotavirus positivity during exacerbated UC as tested by Rapid CER test at different age groupthe results gives (23) positive samples for Rotavirus 46% and negative (27) 54%. The Rotavirus existencein stool of patients with positive biopsy for ulcerative colitis by using chromatography test revealed that thenumber of positive were 20 out of 50 at a rate of 40%. While the RT-PCR product analysis of VP4 gene inRotavirus from in RNA extracted from stool patients samplesthe positive were 24 out of 50 with rate of 48%.