Abstract
Abstract Murine acquired immune deficiency syndrome (MAIDS) caused by the LP-BM5 isolate of Murine Leukemia Virus (MuLV), is an animal model used to study HIV-induced AIDS. After infection with the virus, the MAIDS susceptible C57BL/6 strain becomes immunocompromised and develops symptoms similar to that of humans infected with HIV-1. Conversely, MAIDS resistant BALB/c animals overcome their MuLV infection and gain adaptive immunity against subsequent challenge. Although it has been established that T cells and the genes found in the major histocompatibility complex (MHC) play a vital role in the outcome of MuLV infection, recent studies have identified genes outside the MHC that also play a role in MAIDS susceptibility. One resistance-associated gene, regenerating islet-derived 1 (Reg1), was found to be highly expressed in the lymph node and spleen of recently infected BALB/c mice, with much lower expression seen in the susceptible BL/6 strain. The aim of this study was to design an enzyme-linked immunosorbent (ELISA) assay that could be used to identify the murine Reg1 protein and quantify its concentration in mouse tissue samples. Preliminary results with this assay show up to two-fold more Reg1 protein in the spleens of BALB/c versus BL/6 animals at three days post MuLV infection. Evaluation of lymph node samples and the possible role of this protein in susceptibility to MAIDS are currently under investigation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
