Abstract

1. 1. The synthesis of 3,3′ diiodothyronine (3,3′T 2) from monoiodotyrosine (MIT) in an assay system containing MIT, pyridoxal, Mn 2+, thyroid microsomes, and a H 2O 2-generating system was studied. The yield of 3,3′T 2 was approx. 17% and was only slightly less if the microsomes were preboiled for 20 min. 2. 2. Omission of microsomes from the assay system and chromatography of a butanol extract of the system in butanol-acetic acid-water disclosed a component with R F 0.8 in 15% yield. The formation of this component depended on the presence of Mn 2+, MIT, and pyridoxal. Spectral analysis and other data indicated that it is a metal chelate of Mn 2+ with a Schiff base formed from pyridoxal and MIT. This metal chelate exhibits peroxidase activity which may be primarily responsible for the synthesis of 3,3′T 2 from MIT in the system studied.

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