Abstract
Objective To investigate the role of protein kinase C (PKC) and mito ATP-sensetive potassium channel(mKATP) channels in 0.5 MAC sevoflurane (1.15％) pretreatment preventing intestinal ischemia/reperfusion injury(Ⅰ/RI)in rats.Methods 45rats were randomly divided into five groups (n=9):① Sham-operated group (Sham group); ② The intestinal ischemia/reperfusion group (Ⅱ/R group); ③ 0.5 MAC sevoflurane preconditioning group (APC group); ④ PKC nonspecific blocker Chelerythrine group (CHE group):intraperitoneal injection of CHE 5 mg/kg at 15 min before 0.5 MAC sevoflurane pretreatment; ⑤ mKATP channel blocker 5-Hydroxydecanoic group (5HD group):intraperitoneal injection of 5HD 10 mg/kg at 15 min before 0.5 MAC sevoflurane pretreatment.Three pieces of intestinal tissues were used as following at 120 min after reperfusion:the pathological changes of intestinal tissues structure were observed by HE staining tissue sections and scored by improved Chiu's in the microscope.The expression of cysteinyl aspartate specific proteinase-3 (Caspase-3) was observed by immunohistochemistry.The expressions of B cell lymphoma/lewkmia-2 (Bcl-2) and Caspase-3 in intestinal tissues were detected by Western blotting.Results Chiu's pathological score in APC group (4.0±1.3) was significantly lower than that of Ⅱ/R group (6.7±1.1),CHE (6.8±1.1) and 5HD group (5.8±1.2) (P＜0.01).The latter three groups showed no significant difference (P＞0.05).Compared with sham group,Bcl-2 and Caspase-3expression levels in the other four groups increased significantly (P＜0.01).Compared with APC group,Bcl-2 expression levels in CHE group,5HD group and Ⅱ/R group decreased significantly(P＜0.05).Compared with sham group,Caspase-3 expression levels in the other four groups increased significantly (P＜0.01).The increased level of APC group is significantly less than CHE group,the 5HD group Ⅱ/R group (P＜0.01),the latter three groups showed no significant difference (P＞0.05).Conclusions 0.5 MAC sevoflurane pretreatment could protect against the intestinal Ⅱ/R through activation of PKC and mKATP channel. Key words: Reperfusion injury; Intestines; Sevoflurane; Preconditioning; Protein kinase C ; Mito ATP-sensetive potassium channel
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