Abstract
The aim of this study was to examine the role of prolactin (PRL) in promoting the effects of estradiol (E 2) on the uterus. Ovariectomized rats were given a single injection of E 2 dipropionate (10 μg/rat), along with injections of ovine prolactin (PRL; a single dose — 2.5 IU/rat), or injections of metoclopramide (a single dose — 0.5 mg/rat), or injections of bromocriptine (a single dose — 0.25 mg/rat). The effects of E 2 on the uterus were determined by measuring mitotic indexes; cell, nucleus and nucleolus volumes (morphometry), and DNA content (Feulgen's method) in luminal and glandular epithelia, endometrial stromal cells at 24, 36 and 48 h after the injection of E 2. It is revealed that at each time point after E 2 and PRL or metoclopramide treatment almost all the tested parameters were lower than those in control rats treated with E 2 and distilled water. The values obtained in all the periods after E 2 along with bromocriptine treatment were higher compared with those in E 2-treated animals. Most of the results are statistically significant ( P < 0.05−0.001). In the absence of E 2 none of the drugs used influenced the uterine parameters tested. The effect of PRL is likely to be mediated by their receptors in the uterus, and it occurs by changing the mechanism of estrogen action in the uterus. The actions of metoclopramide and bromocriptine are probably also mediated by PRL.
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