The role of pressure in controlling the entry of water into the developing eggs of the Australian plague locust Chortoicetes terminifera (Walker)

Abstract

AbstractThe normal internal hydrostatic pressure and the additional pressure necessary to rupture the egg shell was measured in the eggs of Chortoicetes terminifera, Newly laid white eggs burst at c 0.15 kg cm‐2, but after external tanning the chorion withstands c 0.5 kg cm‐2 when removed from its tanned foam ‘corset’ and 1.0 kg cm‐2 if left embedded in the egg pod material. Older eggs with formed cuticles often withstand 2.0 kg cm‐2 but yield at rather lower pressures if they develop ‘pin‐holes’. As the OP of the egg contents always exceeds 7.7 kg cm‐2 the rigidity of the wall is clearly insufficient to permit the generation of high hydrostatic pressures capable of preventing water entry during the non‐absorbing phases of development. Real hydrostatic pressures are lower than 0.06 kg cm‐2 in the young intact egg and reach only c 0.5 and 0.3 kg cm‐2, respectively, during the absorptive and post‐absorptive phases of development.Several events contribute to the sigmoid form of the water uptake curve. Water is at first excluded by a permeability barrier associated with the chorion. Absorption is delayed until the yolk is completely enclosed by the serosal cell layer. After undergoing cleavage, the yolk is then rapidly mobilized to furnish precursors for cuticle synthesis; in consequence, the internal OP rises from δ 0.76d̀K to 0.93d̀K despite the massive inflow of water which is governed by the osmotic gradient. At blastokinesis the serosa becomes detached from the cuticle; cuticle deposition and yolk mobilization are halted, the OP falling rapidly to cδT 0.53d̀K. The bulk entry of water then ceases. Any excessive hydrostatic pressures which develop later are relieved by the formation of self‐sealing ‘pin‐holes’.