The role of Prdm12 in adult nociceptors

Abstract

Event Abstract Back to Event The role of Prdm12 in adult nociceptors Aurore Latragna1, 2*, Simon Vermeiren2, Panagiotis Tsimpos2, Laurence Ris1 and Eric Bellefroid2 1 University of Mons, Belgium 2 Free University of Brussels, Belgium Multiple mutations in Prdm12 have been identified in people with congenital insensitivity to pain (CIP). Prdm12 belongs to the Prdm proteins family which are epigenetic regulators involved in neurogenesis (1). Recent results of the laboratory have shown that Prdm12 is selectively expressed in developing nociceptors and that it remains expressed in mature nociceptors in adults. Using Prdm12 KO and cKO mice, Prdm12 was found to be important in developing nociceptors for the initiation and the maintenance of the expression of the NGF receptor TrkA through its ability to modulate the inducing activity of the neurogenin 1 and neurogenin 2 proneural transcription factors (2). Our aim here is to investigate the role of Prdm12 in adult nociceptors and determine whether it plays a role in pain perception. To delete Prdm12 at adult stage specifically in somatosensory neurons of adult mice, we are crossing our Prdm12 floxed mice with Advilin-Cre-ERT2 mice. Prdm12fl/ fl; Advilin-Cre-ERT2 mice will then be injected with tamoxifen to induce the excision of the floxed Prdm12 alleles. Preliminary results of our analysis of the expression of different nociceptive markers in dorsal root and trigeminal ganglia of tamoxifen treated and untreated mice will be presented. On the other hand, DRG cultures have been developed in order to perform subsequent analysis of their electrophysiological properties. Acknowledgements Aurore Latragna is a recipient of a Télévie fellowship. The project is supported by a Win2wal grant of the Wallon Region. References (1) Chen, Y.C., Auer-Grumbach, M., Matsukawa, S., Zitzelsberger, M., Themistocleous, A.C., Strom, T.M., Samara, C., Moore, A.W., Cho, L.T., Young, G.T., et al. (2015). Transcriptional regulator PRDM12 is essential for human pain perception. Nat. Genet. 47, 803–808. (2) Desiderio, S., Vermeiren, S., Van Campenhout, C., Kricha, S., Malki, E., Richts, S., Fletcher, E., Vanwelden, T., Schmidt, B., Henningfeld, K., Pieler, T., Woods, C., Nagy, V., Verfaillie, C. and Bellefroid, E. (2019). Prdm12 Directs Nociceptive Sensory Neuron Development by Regulating the Expression of the NGF Receptor TrkA. Cell Reports, 26(13), pp.3522-3536.e5. Keywords: Pain, Prdm12, nociceptor, epigenetic, DRG - dorsal root ganglion Conference: 13th National Congress of the Belgian Society for Neuroscience , Brussels, Belgium, 24 May - 24 May, 2019. Presentation Type: Poster presentation Topic: Cellular/Molecular Neuroscience Citation: Latragna A, Vermeiren S, Tsimpos P, Ris L and Bellefroid E (2019). The role of Prdm12 in adult nociceptors. Front. Neurosci. Conference Abstract: 13th National Congress of the Belgian Society for Neuroscience . doi: 10.3389/conf.fnins.2019.96.00031 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Apr 2019; Published Online: 27 Sep 2019. * Correspondence: Miss. Aurore Latragna, University of Mons, Mons, Belgium, aurore.latragna@umons.ac.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Aurore Latragna Simon Vermeiren Panagiotis Tsimpos Laurence Ris Eric Bellefroid Google Aurore Latragna Simon Vermeiren Panagiotis Tsimpos Laurence Ris Eric Bellefroid Google Scholar Aurore Latragna Simon Vermeiren Panagiotis Tsimpos Laurence Ris Eric Bellefroid PubMed Aurore Latragna Simon Vermeiren Panagiotis Tsimpos Laurence Ris Eric Bellefroid Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.