The Role of Polymorphism of Regulatory Region of MTDH Gene (Rs1835740) in Migraine and Other Forms of Primary Headaches

Abstract

Objective: There is evidence that MTDH gene has a role in migraine pathophysiology. In our research, association of SNP in MTDH gene (rs1835740) with clinical parameters of migraine is considered. Background: As a result of the first genome-wide association study (GWAS) of a common migraine, a SNP in the regulatory region of MTDH gene (rs1835740) was found. However, the confirmation of GWAS findings on independent samples failed. Also, there is no clear answer on the role of this substitution in the formation of the clinical picture of the various forms of migraine. Patients and methods: The study included 143 patients with migraine. Comparison groups consisted of 9 patients with cluster headache and 20 patients with chronic tension headache. The control group included 362 unexaminated subjects. Genotypes were determined using real-time PCR with allelic discrimination test. Results: Our study evaluated the role of rs1835740 substitution in the clinical picture of the various forms of migraine (episodic, chronic), as well as identified the specificity of this marker for migraine compared with other forms of headache (cluster headache, chronic tension headache). We have not found any effect of T allele (rs1835740) on the formation of the clinical picture of migraine with aura and migraine without aura. Also, we have shown that rs1835740 polymorphism has no effect on the chronification of migraine. Meanwhile, the carriage of T allele is specific for patients with migraine and cluster headache, but is not a characteristic feature of patients with chronic tension headache. Conclusions: Our results suggest that the T allele of substitution rs1835740 in MTDH gene have not effect on the formation of the clinical picture of migraine with aura and migraine without aura, but specific for patients with migraine and cluster headache.