Abstract
When studying the formation of secondary metabolites represented by phenolic compounds in callus cultures of sunflower plants, it was found that under in vitro conditions under the influence of a stress factor, the accumulation of polyphenols increases. It was found that the biosynthetic activity of callus cultures with respect to polyphenol increases in proportion to the concentration of the culture filtrate of the fungus (Sclerotinia Sclerotiorum) in the nutrient medium. This is confirmed by the qualitative and quantitative determination of the content of polyphenols in callus cultures. In savory plants, when introduced into culture in vitro, there is a sharp decrease in the biosynthesis of phenolic compounds. Ethanol extracts of microclones and callus cultures showed no fungicidal activity.
Highlights
Due to the peculiarities of the metabolism of the plant organism, where in addition to the reactions of primary metabolism, a variety of substances of secondary origin are synthesized, the extensive therapeutic effect of plant extracts is manifested
Plant cultures in virto are used in biotechnology as a model object, and as a source of valuable biologically active substances that are successfully used in the pharmaceutical industry, as well as for the study of secondary metabolism [3]
Our studies have shown that culture filtrate (CF) of the fungus Sclerotinia sclerotiorum at different concentrations has a different degree of toxic effect on the germination of sunflower seeds of the studied genotype
Summary
Due to the peculiarities of the metabolism of the plant organism, where in addition to the reactions of primary metabolism, a variety of substances of secondary origin are synthesized, the extensive therapeutic effect of plant extracts is manifested. The most valuable plants for phytopharmacognosia are characterized by a limited range and specific growing conditions and are classified as a small number of endangered species. Plant cultures in virto are used in biotechnology as a model object, and as a source of valuable biologically active substances that are successfully used in the pharmaceutical industry, as well as for the study of secondary metabolism [3]
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