Abstract
IntroductionPiezo1 proteins are large integral proteins with 24‐39 transmembrane domains. Piezos are expressed in several tissues. Piezo ion channel family is evolutionarily conserved in mechanotransduction. We examined the expression level of Piezo1 in rat aortic smooth muscle cells (ASMCs) and determined stretch induced cytosolic calcium rise in these cells.MethodmRNA and protein expressions of piezo 1 and 2 proteins in rat tissues were detected by RT‐PCR and western blot study, respectively. Stretch‐induced cytosolic calcium elevation in RASMC was determined by confocal microscopy.ResultsIn rats, Piezo1 mRNA and protein was highly expressed in aorta, aortic smooth muscle cells and mesenteric artery, but low in cerebral artery, nodose ganglion and petrosal ganglion. Stretch‐induced [Ca2+]i elevation was found to be increased in Piezo1 over‐expressing rat ASMCs compared with native rat ASMCs.ConclusionThe results indicated that Piezo1 may play a functional role in stretch induced [Ca2+]i elevation in rat arterial smooth muscle cell.
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