Abstract
Membrane permeability assays – together with metabolic stability and solubility assays – belong to the essential tools of early drug discovery process. In most therapeutic indications, a drug needs to pass several biological barriers to exert its desired effect to a target tissue, receptor, enzyme, without significant decay. There are numerous models available allowing us to evaluate compounds permeability, with cellular assays being particularly useful. Most frequently, these assays are based on a „Transwell“ system, where two compartments are separated by a filter bearing either artificial membrane or a monolayer of tightly connecting cells. More sophisticated permeability assays have been developed during the last decade, e.g., co-cultures of relevant cell types or 3D microfluidics on a chip. However, their high costs and time-consuming preparation prevent their widespread use. In basic drug development screening cycles Caco-2 and MDCK assays remain the gold standard.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
