The role of pericytes in controlling angiogenesis in vivo.

Abstract

In order to evaluate the interaction between endothelial cells and the perivascular pericytes during physiological angiogenesis, stereological analysis of fine structure was performed on samples of rat skeletal muscle where capillary growth was induced to a similar extent by three different interventions (indirect electrical stimulation, vasodilatation by alpha 1-blockade, stretch due to synergist extirpation). There was a significant reduction in the relative area of contact between pericytes and the capillary abluminal surface with stimulation, and withdrawal of pericyte processes coincided with an increase in anatomical capillary supply. These data indicate that pericytes may play an anti-angiogenic role in vivo in normal adult tissue similar to that proposed for in vitro models of angiogenesis, with their retraction during increased muscle activity possibly releasing endothelial cells from their contact inhibition. However, following long-term peripheral vasodilatation expansion of the capillary bed was accompanied by a co-ordinated increase in pericytes, such that coverage of capillaries was similar to that in control muscles. In addition, growth of capillaries following prolonged stretch resulted in a slightly greater increase in the pericyte population, suggesting they may be permissive for endothelial cell migration. Thus, the role of pericytes in controlling physiological angiogenesis is dependent on the nature of the initial stimulus, suggesting that in vitro data have to be interpreted with caution when discussing the mechanism of capillary growth in vivo.