The role of oxidative stress-mediated apoptosis in the pathogenesis of uric acid nephropathy

Lijuan Yang,Baochao Chang,Yaling Guo,Xueping Wu,Lei Liu

doi:10.1080/0886022x.2019.1633350

Lijuan Yang, Baochao Chang + Show 3 more

Open Access

https://doi.org/10.1080/0886022x.2019.1633350

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Abstract

Objective: By copying the uric acid nephropathy rat model, the oxidative stress injury of mitochondria was caused in renal tubular epithelial cells and the relationship between the injury and the induction of cell apoptosis was identified.Methods: All rats were randomly divided into NC (normal control, NC) group, HUA (high uric acid, HUA) group and GSH (reductive glutathione, GSH) group. The values were quantitatively tested in the kidney tissues, including 24-h urinary protein quantity, serum creatinine, blood uric acid, the MDA (malondialdehyde, MDA) and SOD (superoxide dismutase, SOD) oxidative stress indicators. The expression of p53, Bax and caspase-9/-3 were detected by immunoblotting. TUNEL assays were used to detect the apoptosis of renal tubular epithelial cells.Result: In HUA and GSH groups, the 24-h urinary protein(24UTP), serum creatinine, and blood uric acid increased gradually with the increase of the replication cycle and the increase was significant compared to the NC group (p < .05). Compared to the NC group, MDA increased whereas SOD decreased. The expression of apoptotic proteins, such as p53, Bax, and caspase-9/-3 in the mitochondria was significantly different (p < .05). TUNEL assay revealed that the renal tubular epithelial cells in HUA group were largely apoptotic, whereas the GSH group improved significantly.Conclusion: Mitochondria incurred the substantial damage due to being in a state of oxidative stress, which was the primary cause of apoptosis in the renal tubule epithelial cells. GSH exhibited the effective resistance to the influence of oxidative stress and can restore the damage in the renal tubular epithelial cells.

Highlights

After the treatment of GSH, the serum creatinine and 24UTP were improved in the GSH group compared with the NC and HUA groups (p < .05) (Table 2)
We found that the blood uric acid, blood creatinine and urine protein of the HUA and GSH groups increased gradually compared with the NC group
We found that the MDA of the HUA and GSH groups increased compared with the NC group at the end of the 4th week, and the SOD decreased compared with the NC group

Summary

Methods

2.1. The experimental materialsSprague-Dawley (SD) rats and feed were purchased from Bengbu Medical College Animal Experimental Center. Yeast (purchased from Nanjing Institute of Bioengineering, CHN), adenine (purchased from Nanjing Institute of Bioengineering, CHN), a Mitochondrial extraction kit (purchased from Nanjing Jiancheng institute of Biological Engineering, CHN), MDA and SOD detection reagents (purchased from Nanjing Jiancheng Institute of Biological Engineering, CHN), detection of p53, Bax and caspase-9/-3 antibodies (purchased from Wuhan Seville Biotechnology Co., LTD., CHN), and a TUNEL kit (purchased from Roche, USA) were also used. This study was authorized by the Ethics Committee of Bengbu Medical College, batch no.2015.026.2.2. The experimental method2.2.1. Replica animal model

Results

Discussion

Conclusion