The Role of Ovarian Reserve Tests in predicting Intra-Cytoplasmic Sperm Injection Cycles Outcome

Abstract

Aims: 1. to assess the ovarian reserve (OR) for subpopulations of Iraqi infertile couples seeking Intra Cytoplasmic Sperm Injection (ICSI) treatment. 2. To correlate the OR markers with ICSI outcome. Study design: a prospective randomized controlled trial. Patients and methods: Eighty -seven participants were enrolled during their attendance to fertility center of Al-Sadar Medical Teaching city in Al- Najaf Province; twenty subjects as a control group (n=20), and 67 as a study group (n=67). Ovarian reserve parameters were assessed and ICSI were performed. We correlate the OR markers with: No. of follicles obtained, No. of mature oocytes, Fertilization rate, Cleavage rate, Embryo scoring, No. of embryo transferred and Pregnancy rate (biochemical pregnancy). Results: Age resulted in significant negative correlation with No. of follicles, No. of mature oocytes at metaphase II (MII), No. of two pronuclear phase (2PN),No. of best quality embryos grade one and two (G1+G2),and to the total No. of embryos transferred ;while basal serum level of anti mullerian hormone (AMH) follows age as it shows positive significant correlation with The No. of MII oocytes obtained , No. of best quality embryos ,it correlate positively and significantly to the level of serum AMH. Basal serum level of Follicle stimulating hormone (FSH) shows significant negative correlation with No. of follicles aspirated and to No. of MII oocytes. Basal serum levels of estradiol and inhibin B didn’t show significant correlation with ICSI outcomes. None of the OR markers correlates with pregnancy rate.Conclusion: Age, Anti-Mullerian hormone and follicle stimulating hormone are better markers than inhibin B, estradiol or antral follicle count in predicting the outcome of ICSI cycles.