Abstract

Oxygen-limited continuous cultures of the cowpea Rhizobium sp. strain CB756, had high levels of nitrogenase activity, which were not significantly affected by excess ammonium ions or glutamine. When the growth-restricting O 2-limitation was partially relieved, nitrogenase was repressed and this was accompanied by increased adenylylation of glutamine synthetase. It is suggested that the restricted supply of ATP interferes with adenylylation of glutamine synthetase during O 2-limited growth, thus preventing repression of nitrogenase in the presence of excess ammonium ions.

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