The role of nitrogen in chromophoric and fluorescent dissolved organic matter formation

Abstract

Microbial and photochemical processes affect chromophoric dissolved organic matter (CDOM) dynamics in the ocean. Some evidence suggests that dissolved nitrogen plays a role in CDOM formation, although this has received little systematic attention in marine ecosystems. Coastal seawater incubations were carried out in the presence of model dissolved organic nitrogen (DON: amino sugars and amino acids) and dissolved inorganic nitrogen (DIN) compounds to assess their role in biological and photochemical production of CDOM. For several of the dissolved N compounds, microbial processing resulted in a pulse of CDOM that was mainly labile, appearing and disappearing within 7 days. In contrast, a net loss of CDOM occurred when no N was added to the microbial incubations. The greatest net biological CDOM formation was found upon addition of amino sugars (formation of fluorescent, mostly labile CDOM) and tryptophan (formation of non-fluorescent, refractory CDOM). Photochemical formation of CDOM was only found with tryptophan, the one aromatic compound tested. This CDOM was highly fluorescent, with excitation–emission matrices (EEMs) resembling those of terrestrial, humic-like fluorophores. The heterogeneity in CDOM formation from this collection of labile N-containing compounds was surprising. These compounds are common components of biopolymers and humic substances in natural waters and likely to contribute to microbially- and photochemically-produced CDOM in coastal seawater.