The role of multiple stressors in ranavirus-caused amphibian mortalities in Acadia National Park wetlands

Abstract

Recent studies suggest that multiple sublethal stressors compromise amphibian immune systems and increase susceptibility to disease. We examined two aspects of multiple stressors and incidence of ranavirus-caused amphibian mortalities in free-living amphibian populations: (1) among-pond differences in physical, chemical, and biological stressors that may exacerbate mortality events, and (2) temporal changes in within-pond stressors that coincide with mortality events. At the among-pond scale, we used principal components analysis and logistic regression followed by Akaike’s information criterion (QAICc) to identify stressors associated with disease incidence. Of the stressors we investigated, aluminum, temperature, and conductivity were most correlated with outbreaks, but it was unclear whether they increased ranavirus-caused mortality events. Sublethal stressors were difficult to isolate in the field and few were significantly associated with ranavirus across all breeding ponds. Our results suggest that each wetland, because of varied physical, biological, and chemical settings, will have its own suite of stressors that sublethally affect amphibians.