The Role of Monoamines on MPP+ and H2O2 Induced Oxidative Stress Response

Abstract

Parkinson's Disease is a neurodegenerative disorder characterized by the depletion of monoamines, degeneration of dopaminergic neurons, and subsequent symptoms of bradykinesia, muscle rigidity, and other movement deficiencies. The production of reactive oxygen species has also been implicated in PD. It was previously demonstrated in vivo, that monoamines are depleted in the brain prior to the degeneration of dopamine neurons induced by the Parkinson's inducing agent MPTP. The current study seeks to examine the effects of monoamines such as dopamine, norepinephrine, and serotonin in buffering the effects oxidative stress and cell death mediated by hydrogen peroxide and the Parkinson's inducing agent MPTP/MPP+. The study also aims to elucidate how the monoamines provide this neuroprotective effect. The data has shown differing effects between the different monoamines which may be due to their differing structures. It is hypothesized that they interact with the Nrf2/KEAP1 pathway which regulates the antioxidant enzyme system. The data from these studies will provide potential insight into the role that monoamines play in the neurodegenerative process.Support or Funding InformationThis work was supported by NIH Grant U54NS083932 and NIMHD Grant #5S21MD000101. The investigation was conducted in a facility constructed with support from Research Facilities Improvement Grant #C06 RR‐07571 from the National Center for Research Resources, NIH.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.