The role of molecular genetics in field studies on malaria parasites

Abstract

Molecular genetics is having an important impact on the study of genes in natural populations of malaria parasites. The polymerase chain reaction (PCR) is proving particularly valuable for identifying genes in parasites taken directly from their hosts, without the need to establish them in culture. This is leading to novel methods of diagnosis, for example of drug-resistant parasites. Molecular techniques are also greatly assisting understanding of the genetic structure of parasite populations. This is relevant to the current debate on whether Plasmodium falciparum has a clonal or randomly interbreeding structure. Many patients are infected with mixtures of genetically distinct clones. PCR is being used to examine the genotypes of individual oocysts in the mosquito vector. In wild-caught mosquitoes in areas highly endemic for P. falciparum, a large proportion of oocysts are heterozygous, showing that cross-mating occurs frequently between clones during mosquito feeds. In areas of lower endemicity, there is evidence of less frequent crossing.