Abstract
1. Successful cloning of an adult breeding boar from the novel Chinese Guike No. 1 swine specialized strain Jun-yu Nie, Xiang-xing Zhu, Bing-kun Xie, Su-qun Nong, Qing-yan Ma, Hui-yan Xu, Xiao-gan Yang, Yang-qing Lu, Ke-huan Lu, Yu-ying Liao, Sheng-sheng Lu 3 Biotech CrossRef
Highlights
Cloned mammals produced by nuclear transfer (NT) are genetic duplicates of the donor cell nuclear genome
In the somatic cell nuclear transfer procedure foreign mitochondria are introduced into the enucleated recipient oocyte, clonal offspring may not be genetically identical to the donor cell mitochondrial genome
If nuclear recipient ooplasts are not derived from the same maternal lineage as the donor nuclei a high level of mitochondrial genotype heterogeneity often occurs in clonal embryos and resultant offspring as a result of the variability of specific mtDNA nucleotide sequences (Plante et al, 1992; Campbell, 1999a,b; Steinborn et al, 1998b; Hiendleder et al, 1999, 2004; Smith et al, 2000)
Summary
Cloned mammals produced by nuclear transfer (NT) are genetic duplicates of the donor cell nuclear genome.
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