Abstract
ABSTRACT Aim: To observe the effect of cytokine-induced killer cells (CIK) on the apoptosis of human lymphoma cells (Raji and BJAB), and explore the role of microRNA-155(miR-155) in this process. Methods: MiR-155 was determined by real-time quantitative PCR, and the apoptosis was detected by flow cytometry in Raji and BJAB cells. Psi CHECK2-CEBPB 3′-UTR containing the binding site of miR-155 was constructed, and then transfected into Raji and BJAB cells. Luciferase activity of CEBPB (CCAAT/enhancer binding protein beta) was determined with the assistance of a dual luciferase report system. Results: CIK cells could promote Raji and BJAB cells apoptosis (t = 3.634, P Conclusions: CIK cells could enhance human lymphoma Raji and BJAB cells apoptosis by upregulating miR-155, which may provide a new database to elucidate lymphoma cell therapy using CIK cells. Disclosure: All authors have declared no conflicts of interest.
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