Abstract
Vaso-occlusion in sickle cell anaemia (SCA) is mediated via increased expression of adhesion molecules. Micro-RNA 466i up regulates the expression of interleukin-10 and may contribute to the pathogenesis of the complications in SCA. We sought to investigate the relationship between changes in the level of micro-RNA 466i and the frequency of vaso-occlusive complications in SCA patients. Red blood cells were lysed using ammonium chloride. The resulting white blood cell pellets were lysed in guanidiumisothiocyanate (GITC) lyses buffer. From the GITC lysate, total RNA was extracted. Thereafter, the amount of micro-RNA 466i was quantified. There were no relationships between microRNA 466i and the frequency of complications in SCA patients. (p=0.066) There was also no significant difference in the levels of micro-RNA 466i between patients who had vaso-occlusive complications and those that did not.(p=0.9307) Micro-RNA 466i increased with age (p=0.03) but there was no significant difference between the males and the females patients.(p= 0.370) Micro-RNA 466i (a pro-inflammatory nucleotide) play little or no role in the pathogenesis of vaso-occlusive complications in SCA. Its assay in this group of patients may is not useful in the overall management of these patients.
Highlights
Vaso-occlusion in sickle cell anaemia (SCA) is mediated via increased expression of adhesion molecules
There were no relationships between microRNA 466i and the frequency of complications in SCA patients. (p=0.066) There was no significant difference in the levels of micro-RNA 466i between patients who had vaso-occlusive complications and those that did not.(p=0.9307) Micro-RNA 466i increased with age (p=0.03) but there was no significant difference between the males and the females patients.(p= 0.370) Micro-RNA 466i play little or no role in the pathogenesis of vaso-occlusive complications in SCA
This was higher than the mean age of those without complications (17 ± 10.69 years). This supports the observation that the tendency to develop vaso-occlusive complications of sickle cell anaemia was more after the age of 20 years. This slightly differs from previous studies by Ebong[23] and Iwegbe[24] who indicated that the onset of complications in sickle cell anaemia were higher after the age of 14years
Summary
Vaso-occlusion in sickle cell anaemia (SCA) is mediated via increased expression of adhesion molecules. Microvascular occlusion in the bone marrow leads to hypoxia, tissue ischaemia and infarction, usually characterized by excruciating pains in the long bones, sternum and spine.[18] Vaso-occlusive complications occur due to tissue damage and organ dysfunction results from repeated episodes of blood vessel occlusion by blood cells. These complications include cerebro-vascular accident (stroke), which manifests as motor or sensory deficit; visual impairment or blindness. We undertook this study to investigate the relationship between changes in the level of micro-RNA 466i and the frequency of vaso-occlusive complications in SCA patients
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