Abstract
Background Osteoarthritis (OA) is a common arthritic disease and multifactorial whole-joint disease. Interactions of chemokines and OA is inadequately documented.Results In vivo and in vitro studies were conducted to investigate monocyte chemoattractant protein 1 (MCP-1) and receptor chemokine (C–C motif) receptor 2 (CCR2) in chondrocyte degradation and cartilage degeneration. Chondrocytes from 16 OA patients and 6 normal controls were involved in this study. After stimulation of MCP-1, the expression of MCP-1 and CCR2 increased significantly (P < 0.001) and the expression of MMP-13 also increased (P < 0.05). MCP-1 stimulation also induced (or enhanced) the apoptosis of OA chondrocytes (P < 0.05). Additionally, the degradation of cartilage matrix markers (metalloproteinase 3 and 13, MMP3 and MMP13) in the culture medium of normal chondrocytes was also assessed. Furthermore, intra-articular injection of MCP-1 in mouse knees induced cartilage degradation and the CCR2 antagonist did not impede cartilage destroy in rats knees of monosodium iodoacetate (MIA) model.ConclusionsThe results of this study demonstrate that the MCP-1-CCR2 ligand-receptor axis plays a special role in the initiation and progression of OA pathology. Patients with ambiguous etiology can gain some insight from the MCP-1-CCR2 ligand-receptor axis.
Highlights
Osteoarthritis (OA) is a common arthritic disease and multifactorial whole-joint disease
We investigated the interaction between monocyte chemoattractant protein 1 (MCP-1) and chondrocytes and the possible role of MCP-1-CCR2 ligand-receptor axis in cartilage degradation and disease progression in osteoarthritis
We observed that OA synovia fibroblasts had the highest expression of MCP-1; while CCR2 was mostly expressed in RA synovial fibroblasts (Fig. 1c, d)
Summary
Osteoarthritis (OA) is a common arthritic disease and multifactorial whole-joint disease. OA is a common arthritic disease and multifactorial whole-joint disease. OA is characterized by the degradation of cartilage, as the main (or unique) cell in cartilage; the chondrocyte plays an important role in cartilage degeneration and OA disease pathology [8,9,10]. Despite the cytokines, the chemokines alone may mediate certain independent interactions with chondrocytes and play a role in the pathology of OA. We investigated the interaction between MCP-1 and chondrocytes and the possible role of MCP-1-CCR2 ligand-receptor axis in cartilage degradation and disease progression in osteoarthritis
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