The role of low-density lipoprotein diluents to spermatozoa motility on limousine liquid semen during storage at 5°C

Abstract

The length of the spermatozoa storage process can experience a decrease in quality, so it is not feasible to be disseminated. This study aimed to reduce the declining rate of quality of bovine spermatozoa during storage at 5°C after the addition of egg yolk and Low-Density Lipoprotein (LDL) on diluent medium. The study design used a Completely Randomized Design with a factorial pattern (4 x 5) with replications 3 times, where factor A is an LDL thinner (Al: 20% whole egg yolk (control); A2: 7%; A3: A %; A4: 9%) factor B is the length of storage (B1: first day; B2: second day; B3: third day; B4: fourth day; B4: fifth day). The results showed that the spermatozoa motility of Limousine bovine with low-density lipoprotein diluents had high motility compared to spermatozoa diluted with egg yolk diluents during storage at 5°C, the treatment of low-density lipoprotein can reduce the rate of spermatozoa decreased during storage compared to 20% egg yolk. The best LDL concentration is 8% during storage. The conclusion of this study shows that the administration of LDL could minimize the rate of deterioration in the quality of Limousine bovine spermatozoa during storage compared to egg yolk.