Abstract
The role of free oxygen radicals in experimental acute pancreatitis induced by common bile duct ligation was investigated by measuring malondialdehyde levels in the rat pancreas. Also, the potential role of leucocytes as the source of free oxygen radicals was tested by inducing leukopenia with methotrexate. The malondialdehyde levels in the control, pancreatitis and pancreatitis + methotrexate groups were 9.6 ± 2.0, 44.8 ± 11.4, and 25.6 ± 5.0 nmol malondialdehyde/ g pancreas tissue respectively. The corresponding histopathological severity scores were 0.5 ± 0.7, 8.1 ± 1.2 and 3.7 ± 1.1. The results suggest that the leucocyte may be an important source of free oxygen radicals in this experimental model.
Highlights
Free oxygen radicals (FORs) are metabolic by-products which are rapidly inactivated by scavenger systems
Sanfey et al have shown that FORs play a role especially in early pancreatitis and have hypothesized that, FORs, which are increased in all pancreatitis models, originate from pancreatic chymotrypsinogen activity and xanthine dehydrogenase (XD)-derived xanthine oxidase (XO)[1]
In this study we investigated the role of leucocytes in acute pancreatitis induced by common bile duct ligation
Summary
Hepatopancreatobiliary Surgery Unit, Department of General Surgery Istanbul Faculty of Medicine, Istanbul, Turkey (Received 10 January 1994). The role of free oxygen radicals in experimental acute pancreatitis induced by common bile duct ligation was investigated by measuring malondialdehyde levels in the rat pancreas. The potential role of leucocytes as the source of free oxygen radicals was tested by inducing leukopenia with methotrexate. The malondialdehyde levels in the control, pancreatitis and pancreatitis + methotrexate groups were 9.6 __+ 2.0, 44.8 + 11.4, and 25.6 5.0 nmol malondialdehyde/g pancreas tissue respectively. The results suggest that the leucocyte may be an important source of free oxygen radicals in this experimental model
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