The role of ionic transport in the respiratory burst activation in human polymorphonuclear granulocytes

Abstract

The role of ionic transport in the respiratory burst activation in polymorphonuclear granulocytes was investigated by measuring of superoxide anion production by the cells stimulated with 10−7 M FMLP varying the ionic composition of the suspending medium (KRPD). It has been found that the external cationic concentration change had no significant effect on spontaneus and FMLP stimulated superoxide anion production of granulocytes measured continously by recording ferricytochrome c reduction. However inhibition of Na+-K+--pump by Ouabain in the concentration of 10−4 M resulted slight decrease in superoxide anion production of granulocytes stimulated with FMLP. Replacement of external chloride anion with saccharose or blocking the chloride influx across the plasma membrane by Antracenum hydrochloricum in the concentration of 5 × 10−3 M resulted significant decrease of FMLP stimulated superoxide anion production measured by ferricytochrome c or nitrablue tetrasolium reduction. It has been conclude that the extra- and/or intracellular chloride anion can influence on the respiratory burst activity of FMLP stimulated human neutrophil granulocytes.