The role of inflammation in ovarian pathology as a predictor of the development of sterility and its correction by inositol medications

Abstract

Relevance of the research. Ovarian inflammation may be important in the pathology of female reproductive health and may be a predictor of infertility.The purpose of the work. To assess the role of pro- and anti-inflammatory cytokines in the development of inflammation in the ovaries of female rats and to carry out correction with inositol preparations (In); to conduct a retrospective analysis of infertility and its relationship with hormone content.Material and Methods. Experiments were performed on 108 white female Wistar rats, which were divided into 3 series: 1 – control, 3.5-4-month-old rats, 2 – 7.5-8-month-old rats (Age), 3 – hypodynamic stress (Stress). There were 3 groups of animals in each series: A – control, B – inositol (In), B – FT500plus (FT). In and FT were administered intragastrically for 15 days at the rate of 400 mg/kg inositol. The concentration of interleukins (IL) 1beta, 2, 4 and 10 was determined in the blood serum of the animals. A morphological study of the ovaries was carried out. We retrospectively analyzed 137 outpatient records of patients with infertility in the setting of reduced ovarian reserve.Results. Hyperemia of the ovaries was noted in 1 series of animals administered inositol medications. In 1 series of animals, the introduction of In caused an increase in the content of IL-2, and the drug FT led to a decrease in IL-1. Both Age and Stress caused an increase in IL-1beta, and only after hypodynamic stress an increase in IL-10 was noted. When comparing the groups of the 1st series, it was proven that the use of FT for preventive purposes had a greater effect on the body than In. When comparing the results of the 2 series groups, it was found that In and FT had a positive effect on the rats, causing a decrease in pro-inflammatory cytokines and inflammation. When analyzing the groups of 3 series, it was found that with prolonged hypodynamic stress, both In and FT had a positive effect on reducing the inflammatory response in rats.Conclusion. Morphologically, it was established that the use of inositol medications (at the rate of 400 mg/kg) has a positive effect on the morphofunctional state of the ovaries of rats, of rats of all groups. Intragastric administration of FT500plus has a more pronounced effect on the growth and maturation of ovarian cortical follicles compared to inositol.