The Role of IL-10 Gene in Abortion Incidence at the Last Pregnancy Trimester in Iraqi Women

Abstract

Background: The term "abortion" refers to the removal or evacuation of an embryo or fetus to end a pregnancy. As previously mentioned, there is a relation between the IL-10 gene and abortion. Material and method: Genomic DNA was isolated from 100 samples of whole blood obtained from the vein included (50 individuals with mischarged women and 50 control individuals) with biological data collected from participants in this study. An allele-specific PCR technique used to amplify A>G (rs1800896). Results: The findings of the current study shown in Table 3 showed a clear significant difference in the parameter levels in the abortion study group. Based on the genotype frequency, the serum levels of IL-10 AA, AG, and GG increased significantly in the abortion group compared with the control (9.635.3 versus 2.0981.02 p-value 0.001), (15.363.64 versus 2.150.53 p-value 0.001), and (8.615.24 versus 2.190.72 p-value 0.002). The findings of blood pressure related to rs1800896 AA, AG, and GG genotypes in systolic blood pressure were (132.661.99 versus 1200 p-value 0.001), (133.071.34 versus 1200 p-value 0.0001), and (132.341.86 versus 1200 p-value 0.001), while in diastolic blood pressure were (90.951 versus 800, P-value 0.005),(91.341.52 versus 800 p-value 0.002), and (91.021.11 versus 800 p-value =0.001) compared with control. Conclusion: IL-10 rs181206 genetic polymorphism is associated with an increased risk of third trimester miscarriage, and also observed significant differences in IL-10 levels among different genotypes.