Abstract
Background and Aim:Recently, many medicinal plants have received considerable attention in the medical field because of their role in the wound healing potential. This study aimed to determine the effectiveness of H. tuberosus powder on the healing pathway of full-thickness cutaneous wounds in a mouse model.Materials and Methods:H. tuberosus powder was prepared by a freeze-drying process using a lyophilizer and its active ingredients were evaluated by high-performance liquid chromatography (HPLC), while its antibacterial properties were evaluated by agar well diffusion assay. The percentage wound contraction was also assessed. Thirty mice were used, which were divided equally into two groups, a control group and a treated group. A full-thickness wound, 1 cm×1 cm in size, was established on the dorsal aspect of the thoracolumbar region, into which H. tuberosus powder was topically applied in the treated group. In contrast, the control group was left without any treatment. The animals were euthanized on days 7, 14, and 21 after wounding for histopathological study.Results:The agar well diffusion method indicated the antibacterial activity of H. tuberosus, while the HPLC results indicated that the active ingredients of H. tuberosus powder mainly consisted of three major kinds of fatty acid. In addition, the macroscopic results of wound contraction rate and the histopathological outcomes of the healing process were significantly (p≤0.05) enhanced in the treated group compared with those in the control group.Conclusion:H. tuberosus powder acts as an antibacterial agent with the ability to enhance the wound healing process.
Highlights
The healing of cutaneous wounds is a complex biological process by which skin repairs itself after injury
Materials and Methods: H. tuberosus powder was prepared by a freeze-drying process using a lyophilizer and its active ingredients were evaluated by high-performance liquid chromatography (HPLC), while its antibacterial properties were evaluated by agar well diffusion assay
The agar well diffusion method indicated the antibacterial activity of H. tuberosus, while the HPLC results indicated that the active ingredients of H. tuberosus powder mainly consisted of three major kinds of fatty acid
Summary
The healing of cutaneous wounds is a complex biological process by which skin repairs itself after injury. This process consists of three highly integrated steps: Inflammation, tissue proliferation, and tissue remodeling It involves the activation of inflammatory cells, endothelial cells, keratinocytes, and fibroblasts, along with the synthesis and liberation of active mediators (cytokines and growth factors) to regulate tissue repair and regeneration [1]. These steps and their physiological constituents should occur in the appropriate sequence and at certain times to achieve optimal function and the tensile strength of wounded area of the original skin [2]. This study aimed to determine the effectiveness of H. tuberosus powder on the healing pathway of full-thickness cutaneous wounds in a mouse model
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