Abstract
Self-incompatibility is exhibited by callose deposition in the stigmatic papillae and total inhibition of pollen germination in sporophytic self-incompatible (SSI) systems of Ipomoea cairica (Convolvulaceae), Brassica campestris and Raphanus sativus (Brassicaceae). This has been partially overcome, in vitro by treating pollen and/or stigma with gibberellic acid (GA3). Experiments employing tryptophan and chlorocholine chloride either alone or in combination with GA3 (10−3 M) further elucidate the role of gibberellin in overcoming the barrier to self-compatibility. These results support our earlier contention [7, 24] that growth regulators play a significant role in pollen-pistil recognition in SSI systems.
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