Abstract
Abstract During storage of blood in acid-citrate-dextrose (ACD) 2,3-diphosphoglycerate (2,3-DPG) and other organic phosphates are lost from the erythrocytes. This impairs the ability of the blood to release oxygen. When the stored blood is incubated with inorganic phosphate, inosine, and adenosine there is a resynthesis of organic phosphates in the cells with concomitant improvement in the oxygen-binding property of blood. However, a major part of the phosphate is incorporated into hexose diphosphate and triose phosphate giving concentrations of these compounds much higher than those in fresh blood. Addition of either pyruvate or fructose to the incubation medium results in enhanced synthesis of 2,3-DPG and ATP giving a distribution of organic phosphate similar to that in fresh blood. The addition of glucose had no effect. This suggests that fructose can be metabolized in the red cell by a nonglycolytic pathway. Evidence for the conversion of fructose to sorbitol by NAD-linked sorbitol dehydrogenase is presented.
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