Abstract
Fructophilic lactic acid bacteria (FLAB) isolated from honeybee’s nectar stomach represent an important source of antimicrobial substances that has been focused on nowadays. In this study, these FLABs were tested for their antibacterial activity in vivo as well as the detection of Interleukine -17 in the serum of rabbits ingested with FLAB isolates. Fructobacillus fructosus FH1, Fructobacillus fructosus FH8 and Lactobacillus kunkeei SHGH-7 FLAB isolates were used against wound infections caused by Pseudomonas aeruginosa and Staphylococcus aureus (MRSA) in rabbit skin . Enzyme linked sorbent immunoassay (ELISA) was used to detect the levels of Interleukine-17 in the sera of rabbits ingested with these three FLAB isolates. The results showed that the time of wound healing process was shorter in the FLAB treated wounds when compared to control untreated wounds with a significant difference at p≤0.01. Histopathological examination of wounds treated with FLAB isolates showed normal thickness in skin layers with no pathological effect, as well as tissue repair and presence of new blood vessels when compared to pathogen groups that were infected with pathogenic bacteria without any treatment, which showed hyperplasia with necrosis in tissue. Results of the FLAB-ingested groups showed an increase in the level of IL-17 when compared to the control with no statistical significance (P≤0.05). To the best of our knowledge, this is the first study on using FLAB microorganisms in the treatment of infected wounds.
Highlights
Honeybees are very important insects especially for humans, because of their increased value for honey production and for their importance economically and as an ecosystem manager [1]
Interleukin-17 was detected by sandwich Enzyme linked sorbent immunoassay (ELISA) in the serum of rabbits that had been ingested with fructophilic lactic acid bacteria (FLAB), pathogenic bacteria and in the control group
Wounds with 1cm diameter were made on the skin of rabbits to test the activity of three types of FLAB isolates on wounds management
Summary
Honeybees are very important insects especially for humans, because of their increased value for honey production and for their importance economically and as an ecosystem manager [1]. The Test groups were treated by FLAB bacteria (HN-F1, HN-F8 and HN-L7) from Day zero (Day zero here means 2-3 days after each inflammation has occurred for each pathogen used) until they were completely healed, and a biopsy was taken from each rabbit. Interleukin-17 was detected by sandwich ELISA in the serum of rabbits that had been ingested with FLAB, pathogenic bacteria and in the control group.
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