Abstract
Background:Procarbazine (PCZ) is an effective chemotherapeutic drug used in the treatment of lymphoma; however, oxidative stress–mediated testicular toxicity is a major side effect. Recently, therapeutic intervention using flavonoids against oxidative stress–related pathologies is gaining more attention. Morin (MOR) is a natural flavonoid with proven antioxidant activity. This study was designed therefore to evaluate the potential role of MOR in ameliorating PCZ-induced testicular oxidative stress and altered sperm quality in rat model.Methods:A total of 24 male Wistar rats (170–180 g) were randomly assigned into 4 treatment groups: I, control; II, PCZ (2 mg/kg b.w.); III, PCZ (2 mg/kg b.w.) + MOR (100 mg/kg b.w.) simultaneously administered and IV, MOR (100 mg/kg b.w.), and all treatments lasted 14 days.Results:PCZ treatment displayed significant reduction in sperm number, sperm motility, percentage normal sperm cells, and daily sperm production rate. Meanwhile the activities of testicular enzymes: gamma-glutamyl transferase, acid phosphatase, and lactate dehydrogenase were significantly altered in the PCZ group compared to control. Furthermore, PCZ caused a significant reduction in levels of glutathione and ascorbic acid as well as activities superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase in the testes of PCZ-treated rats. A significant increase in testicular malondialdehyde level was also observed in the PCZ group. MOR treatment, however, significantly restored the altered sperm parameters and biochemical markers in the testis.Conclusions:Our data suggest that MOR administration protected against PCZ-induced testicular and spermatotoxicity in rat, by improving testicular antioxidant system.
Highlights
In recent years, chemotherapy has improved the survival rates in many cancer cases such as acute lymphoblastic leukemia, Hodgkin disease, and testicular tumor.[1]
The following substances were employed in the study: PCZ hydrochloride (Naprod Life Sciences Pvt Ltd, Mumbai, India); MOR hydrate, thiobarbituric acid (TBA), GSH, 1-chloro-2,4dinitrobenzene, epinephrine, 5’,5’-dithio-bis-2-nitrobenzoic acid, para-nitrophenyl phosphate, and hydrogen peroxide (Sigma Chemical Company, London, UK)
Administration of MOR significantly ameliorated the alterations in sperm parameters induced by PCZ
Summary
Chemotherapy has improved the survival rates in many cancer cases such as acute lymphoblastic leukemia, Hodgkin disease, and testicular tumor.[1]. Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article. Procarbazine (PCZ) is an effective chemotherapeutic drug used in the treatment of lymphoma; oxidative stress–mediated testicular toxicity is a major side effect. Therapeutic intervention using flavonoids against oxidative stress– related pathologies is gaining more attention. Morin (MOR) is a natural flavonoid with proven antioxidant activity. This study was designed to evaluate the potential role of MOR in ameliorating PCZ-induced testicular oxidative stress and altered sperm quality in rat model
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
