Abstract
The initial rate of formation of insoluble immune complexes from rabbit IgG and ovalbumin was approximately 12 times that for formation of F(ab') 2-ovalbumin complexes. At low IgG concns, in the range 0.7–2.7 n M, the formation of insoluble immune complexes was characterised by a lag phase, especially for complexes formed in low antigen excess, compared to antibody excess. Guanidine HC1 (at concns up to 0.5 M) and urea (at concns up to 1 M) decreased the initial rates of formation of IgG immune complexes more than F(ab') 2 immune complexes. Pre-formed IgG immune complexes were solubilised at lower guanidine HC1 concns than were F(ab') 2 immune complexes. Clq enhanced the initial rate of formation of IgG immune complexes at Clq:IgG ratios up to 1:1. Higher Clq concns decreased the initial rate of formation of the complexes. Urea (1 M) blocked the Clq mediated enhancement of immune complex formation.
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