Abstract
Background: This study was conducted to evaluate the role of exogenous epidermal growth factor (EGF) injection on the Ki-67 immuno-expression in submandibular salivary gland tissue of rats receiving doxorubicin (DXR). Methods: A total of 21 two-month-old male albino rats, of 200 g body weight, were divided into three groups: control group; DXR group, the rats received 20 mg/kg body weight DXR as a single intra peritoneal injection; DXR+EGF group, the rats received the same dose of DXR and on the next day they were injected intraperitoneally with 10 µg/kg body weight of EGF daily for one week. Histological sections and immunohistochemical expression of Ki67 sections were examined using a ZEISS Primo Star light microscopy and images taken using Tucsen IS 1000 10.0MP Camera. Results: Ki-67 expression was significantly increased in submandibular salivary glands of rats after DXR injection. However, Ki-67 expression in the glandular tissue was restored to normal levels after EGF injection. Conclusions: EGF preserved glandular architecture after DXR injection and maintained Ki-67 immune-expression within the glandular tissue near to the normal level.
Highlights
Ki-67 is a heavy protein of 395 kDa weight
Ki-67 is an important protein for cell division as antisense nucleotides of Ki-67 will stop the division, and it is a vital factor in the formation of ribosomes (Schluter et al, 1993)
Histological examination Histological examination of the submandibular salivary gland of rats of control group revealed its main structural components was composed of parenchymal tissue supported by connective tissue stroma (Figure 1A)
Summary
Ki-67 is a heavy protein of 395 kDa weight It is a proliferation marker which is highly expressed in various tumors, and has been used for investigations of many cancer types. Ki-67 is an important protein for cell division as antisense nucleotides of Ki-67 will stop the division, and it is a vital factor in the formation of ribosomes (Schluter et al, 1993) This is reinforced by the conclusion that Ki-67 immuno-expression associates with the rate of protein production and function of ribosomes (Plaat et al, 1999). This study was conducted to evaluate the role of exogenous epidermal growth factor (EGF) injection on the Ki-67 immuno-expression in submandibular salivary gland tissue of rats receiving doxorubicin (DXR). Conclusions: EGF preserved glandular architecture after DXR injection and maintained Ki-67 immune-expression within the glandular tissue near to the normal level
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